CWRU researchers discover byproducts from bacteria awaken dormant T-cells and HIV viruses
5-Jan-2015 - Dental and medical researchers from Case Western Reserve University found another reason to treat periodontal disease as soon
They discovered that byproducts of bacteria in gum disease, called metabolic small chain fatty acid (SCFA), can work together to wake
up HIV in dormant T-cells and cause the virus to replicate.
Their findings help explain why people with the HIV -infections and periodontal disease have higher levels of the virus in their saliva
than HIV patients with healthy gums.
The researchers speculate that byproducts from other bacteria infections in other diseases might change gene expression using similar
For dental patients with HIV, their findings further support how important it is to treat bacterial infections in gum disease early.
This interaction by SCFA and T-cells surprised co-investigators Fengchun Ye, assistant professor of biological sciences at the Case
Western Reserve University School of Dental Medicine, and Jonathan Karn, director of the Center for Aids Research and professor and
chair of the Department of Molecular Biology and Microbiology at Case Western Reserve's medical school.
Their findings are described in the article, "Short chain fatty acids potently induce latent HIV-1 in T-cells by activating P-TEFb and
multiple histone modifications," published in January 2015 in the journal Virology (Das B, et al. Virology . 2015
Jan 1;474:65-81. doi: 10.1016/j.virol.2014.10.033. Epub 2014 Nov 14).
In the interaction between gum disease and HIV, five SCFA byproducts from two prevalent oral bacteria-- Porphyromonas gingivalis (Pg) and
Fusobacterium nucleatum (Fn)--are involved in activating resting immune T-cells carrying latent (inactive) HIV-1 virus.
The process acts much like the jumper cables attached to a live battery recharging a dead one to get it running again, according the
Ye explained that all humans have a reservoir of resting T-cells that wake up and respond to inflammation to ward off an infection in
"As long as someone is healthy, the reservoir remains untapped," he said.
But for people with HIV, these T-cells can also have the sleeper HIV-1 virus, which remains in a dormant state until awakened, Karn said.
Last year, Ye and Karn discovered that one SCFA--butyric acid--induced a chain of events that reactivate the virus associated with
Kaposi's sarcoma, the most common malignancy in HIV patients.
Following that discovery, the researchers expanded their investigation to all SCFAs. They found that a high quantity of butyric acid
the T-cell and incites virus replication. But smaller amounts of the five SCFA, working together, have the same impact.
"Looking at only butyric acid was misleading," said Karn, the Reinberger Professor of Molecular Biology. "It surprised us to find they
all work as an aggregate."
The impact on waking up T-cells and activating HIV replication was a "double whammy" find that contributes to understanding the
little-known microbiome in HIV disease, Karn said.
That prompted the researchers to investigate the mechanism that drives the replication of the virus in gum disease.
HIV antiviral therapy prevents active HIV cells from replicating and doesn't affect the quiet viruses in sleeping T-cells.
As long as the patient is free of gum disease, the virus sleeps and remains in check, Karn said.
Also contributing to the study were CWRU researchers: Biswajit Das and Curtis Dobrowolski, from the Department of Molecular Biology
and Microbiology; Abdel-Malek Shahir and Nabil Bissada, from the Department of Periodontics; and Zhimin Feng, Xiaolan Yu, and Jinfeng
Sha and Aaron Weinberg from the Department of Biological Sciences.
The National Institute of Dental and Craniofacial Research (R56DE023912) and CWRU's Center for Aids Research supported the study.
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